Hairy Root Transformation Using Agrobacterium rhizogenes as a Tool for Exploring Cell Type-Specific Gene Expression and Function Using Tomato as a Model1[W][OPEN]
نویسندگان
چکیده
Department of Plant Biology (M.R., K.K., K.Z., J.G., S.W., H.M., S.I., N.S., S.M.B.) and Genome Center (M.R., K.K., J.G., S.W., H.M., S.I., S.M.B.), University of California, Davis, California 95616; Center for Plant Cell Biology, Botany and Plant Sciences Department, University of California, Riverside, California 92521 (G.P., M.A.R., J.B.-S.); Department of Biology, Emory University, Atlanta, Georgia 30322 (D.W., R.B.D.); Department of Integrated Genetics, National Institute of Genetics, Mishima 411–8540, Japan (S.I.); and Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, United Kingdom (F.F.)
منابع مشابه
Breakthrough Technologies Hairy Root Transformation Using Agrobacterium rhizogenes as a Tool for Exploring Cell Type-Specific Gene Expression and Function Using Tomato as a Model1[W][OPEN]
Department of Plant Biology (M.R., K.K., K.Z., J.G., S.W., H.M., S.I., N.S., S.M.B.) and Genome Center (M.R., K.K., J.G., S.W., H.M., S.I., S.M.B.), University of California, Davis, California 95616; Center for Plant Cell Biology, Botany and Plant Sciences Department, University of California, Riverside, California 92521 (G.P., M.A.R., J.B.-S.); Department of Biology, Emory University, Atlanta,...
متن کاملHairy root transformation using Agrobacterium rhizogenes as a tool for exploring cell type-specific gene expression and function using tomato as a model.
Agrobacterium rhizogenes (or Rhizobium rhizogenes) is able to transform plant genomes and induce the production of hairy roots. We describe the use of A. rhizogenes in tomato (Solanum spp.) to rapidly assess gene expression and function. Gene expression of reporters is indistinguishable in plants transformed by Agrobacterium tumefaciens as compared with A. rhizogenes. A root cell type- and tiss...
متن کاملIn vitro production of secondary metabolite using Atropa komarovii Bline&Shal (Solanaceae) hairy root culture via Agrobacterium rhizogenes ATCC15834
Background & Aim:A new sustainable tissue-based system is presented by plant hairy roots, preserving all of the several specialized types of cell with critical roles in allowing bioactive secondary molecules to be synthesized more consistently as usual. The system is also essential for studying the production of alkaloid in culture. Experimental: The ...
متن کاملIn vitro production of secondary metabolite using Atropa komarovii Bline&Shal (Solanaceae) hairy root culture via Agrobacterium rhizogenes ATCC15834
Background & Aim:A new sustainable tissue-based system is presented by plant hairy roots, preserving all of the several specialized types of cell with critical roles in allowing bioactive secondary molecules to be synthesized more consistently as usual. The system is also essential for studying the production of alkaloid in culture. Experimental: The ...
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Hairy roots culture is a potential system for secondary metabolite production in medicinal plants. In this research, hairy root induction in Echinacea angustifolia was established using Agrobacterium rhizogenes. The effects of dif-ferent A. rhizogenes strains (AR15834, A4, K599 and MSU440) in two solid growth regula-tor-free culture media, half strength MS salts with B5 vitamins and WPM, were e...
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